Assessment of Benzimidazole Resistance Status in an Organized Goat Farm by Egg Hatch Assay
نویسندگان
چکیده
Regular monitoring of benzimidazole resistance status helps in timely implementation of resistance mitigation strategies in farms. Egg hatch assay (EHA) evaluates the ability of benzimidazoles to inhibit or prevent embryonation and hatching of nematode eggs and is the standard in vitro test for detection of benzimidazole resistance. The present study reports detection of benzimidazole resistance in an organized goat farm in Kerala by EHA. Log-probit analysis revealed ED50 of 0.25 μg/ ml thiabendazole which indicated benzimidazole resistance in the farm. The assay was also interpreted using the discriminating dose criterion. Hatching ratio of strongyle eggs (Hdd) at the discriminating dose of 0.1 μg/ml was 0.62 indicating benzimidazole resistance. EHA was found to be a rapid, economic and convenient detection method suitable for routine evaluation of benzimidazole resistance. Detection of benzimidazole resistance in the farm warrants implementation of strategies to control further progression of resistance.
منابع مشابه
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